Yu, Q, Mishra, B and Fein, JB (2020) Role of Bacterial Cell Surface Sulfhydryl Sites in Cadmium Detoxification by Pseudomonas putida. Journal of Hazardous Materials, 391. 122209. ISSN 0304-3894
Abstract
Understanding bacterial metal detoxification systems is crucial for determining the environmental impacts of metal pollution and for developing advanced bioremediation and water disinfection strategies. Here, we explore the role of cell surface sulfhydryl sites in bacterial detoxification of Cd, using Pseudomonas putida with surface sulfhydryl sites mostly on its EPS molecules as a model organism. Our results show that 5 and 20 ppm Cd in LB growth medium affects the lag phase of P. putida, but not the overall extent of cell growth at stationary phase, indicating that P. putida can detoxify Cd at these concentrations. EXAFS analysis of Cd bound to biomass from the different growth stages indicates that Cd binds to both sulfhydryl and non-sulfhydryl sites, but that the importance of Cd-sulfhydryl binding increases from early exponential to stationary phase. Cell growth is positively correlated to the measured sulfhydryl concentration on different biomass samples, but is independent of the measured non-sulfhydryl binding site concentration on the cell surfaces. Taken together, our results demonstrate that the sulfhydryl binding sites on EPS molecules can play an important role in binding and detoxifying toxic metals, significantly decreasing the bioavailability of the metal by sequestering it away from the bacterial cells.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2020, published by Elsevier. All rights reserved. This is an author produced version of an article published in Journal of Hazardous Materials. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Sulfhydryl sites; P. putida; Toxicity; Cd |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Feb 2020 16:06 |
Last Modified: | 30 Jan 2021 01:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jhazmat.2020.122209 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:156572 |
Download
Filename: Role of Bacterial Cell Surface Sulfhydryl Sites in Cadmium Detoxification by Pseudomonas putida.pdf
Licence: CC-BY-NC-ND 4.0