Jia, X, Su, H, Chen, X et al. (8 more authors) (2020) A critical role of the KCa3.1 channel in mechanical stretch‐induced proliferation of rat bone marrow‐derived mesenchymal stem cells. Journal of Cellular and Molecular Medicine, 24 (6). pp. 3739-3744. ISSN 1582-1838
Abstract
Mechanical stimulation is an important factor regulating mesenchymal stem cell (MSC) functions such as proliferation. The Ca2+‐activated K+ channel, KCa3.1, is critically engaged in MSC proliferation but its role in mechanical regulation of MSC proliferation remains unknown. Here, we examined the KCa3.1 channel expression and its role in rat bone marrow‐derived MSC (BMSC) proliferation in response to mechanical stretch. Application of mechanical stretch stimulated BMSC proliferation via promoting cell cycle progression. Such mechanical stimulation up‐regulated the KCa3.1 channel expression and pharmacological or genetic inhibition of the KCa3.1 channel strongly suppressed stretch‐induced increase in cell proliferation and cell cycle progression. These results support that the KCa3.1 channel plays an important role in transducing mechanical forces to MSC proliferation. Our finding provides new mechanistic insights into how mechanical stimuli regulate MSC proliferation and also a viable bioengineering approach to improve MSC proliferation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | bone marrow‐derived mesenchymal stem cells; cell proliferation; KCa3.1 channel; mechanical stretch |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Jan 2020 15:30 |
Last Modified: | 25 Jun 2023 22:07 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/jcmm.15014 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:155841 |