Milligan, CJ and Jiang, L-H orcid.org/0000-0001-6398-0411 (2019) Automated Planar Patch-Clamp Recording of P2X Receptors. Methods in Molecular Biology, 2041. pp. 285-300. ISSN 1064-3745
Abstract
P2X receptors are a structurally and functionally distinctive family of ligand-gated ion channels that play important roles in mediating extracellular adenosine 5′-triphosphate (ATP) signaling in diverse physiological and pathophysiological processes. For several decades, the “manual” patch-clamp technique was regarded as the gold standard assay for investigating ion channel properties. More recently, breakthroughs in the development of automated patch-clamp technologies are enabling the study of ion channels, with much greater throughput capacities. These automated platforms, of which there are many, generate consistent, reliable, high-fidelity data. This chapter demonstrates the versatility of one of these technologies for ligand-gated ion channels, with a particular emphasis on protocols that address some of the issues of receptor desensitization that are commonly associated with P2X receptor-mediated currents.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © Springer Science+Business Media, LLC, part of Springer Nature 2020. This is an author produced version of an article published in Methods in Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Automated electrophysiology; Planar patch-clamp; Planar chip; Microfluidics; Stacked solution application; Ligand-gated ion channels; P2X receptors; Voltage-clamp |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Dec 2019 10:22 |
Last Modified: | 24 Oct 2020 00:39 |
Status: | Published |
Publisher: | Springer Nature |
Identification Number: | 10.1007/978-1-4939-9717-6_21 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:154716 |