Dimitrova, A, Walko, M orcid.org/0000-0002-7160-6136, Hashemi Shabestari, M et al. (3 more authors) (2016) In situ, Reversible Gating of a Mechanosensitive Ion Channel through Protein-Lipid Interactions. Frontiers in Physiology, 7 (SEP). 409. ISSN 1664-042X
Abstract
Understanding the functioning of ion channels, as well as utilizing their properties for biochemical applications requires control over channel activity. Herein we report a reversible control over the functioning of a mechanosensitive ion channel by interfering with its interaction with the lipid bilayer. The mechanosensitive channel of large conductance from Escherichia coli is reconstituted into liposomes and activated to its different sub-open states by titrating lysophosphatidylcholine (LPC) into the lipid bilayer. Activated channels are closed back by the removal of LPC out of the membrane by bovine serum albumin (BSA). Electron paramagnetic resonance spectra showed the LPC-dose-dependent gradual opening of the channel pore in the form of incrementally increasing spin label mobility and decreasing spin-spin interaction. A method to reversibly open and close mechanosensitive channels to distinct sub-open conformations during their journey from the closed to the fully open state enables detailed structural studies to follow the conformational changes during channel functioning. The ability of BSA to revert the action of LPC opens new perspectives for the functional studies of other membrane proteins that are known to be activated by LPC.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Dimitrova, Walko, Hashemi Shabestari, Kumar, Huber and Kocer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | MscL, reversible gating, mechanosensation, electron spin resonance spectroscopy, lysophosphatidylcholines, bovine serum albumin |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Dec 2019 11:42 |
Last Modified: | 04 Dec 2019 11:42 |
Status: | Published |
Publisher: | Frontiers Media |
Identification Number: | 10.3389/fphys.2016.00409 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:154079 |