Tong, Meixuezi, Lee, Kyounghee, Ezer, Daphne et al. (9 more authors) (2020) The Evening Complex establishes repressive chromatin domains via H2A.Z deposition. Plant Physiology. pp. 612-625. ISSN 0032-0889
Abstract
The Evening Complex (EC) is a core component of the Arabidopsis (Arabidopsis thaliana) circadian clock, which represses target gene expression at the end of the day and integrates temperature information to coordinate environmental and endogenous signals. Here we show that the EC induces repressive chromatin structure to regulate the evening transcriptome. The EC component ELF3 directly interacts with a protein from the SWI2/SNF2-RELATED (SWR1) complex to control deposition of H2A.Z-nucleosomes at the EC target genes. SWR1 components display circadian oscillation in gene expression with a peak at dusk. In turn, SWR1 is required for the circadian clockwork, as defects in SWR1 activity alter morning55 expressed genes. The EC-SWR1 complex binds to the loci of the core clock genes PSEUDO56 RESPONSE REGULATOR7 (PRR7) and PRR9 and catalyzes deposition of nucleosomes containing the histone variant H2A.Z coincident with the repression of these genes at dusk. This provides a mechanism by which the circadian clock temporally establishes repressive chromatin domains to shape oscillatory gene expression around dusk.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Society of Plant Biologists. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 26 Nov 2019 15:00 |
Last Modified: | 16 Oct 2024 16:13 |
Published Version: | https://doi.org/10.1104/pp.19.00881 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1104/pp.19.00881 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:153864 |