Zougman, A orcid.org/0000-0002-7168-1339, Wilson, JP, Roberts, LD orcid.org/0000-0002-1455-5248 et al. (1 more author) (2020) Detergent-Free Simultaneous Sample Preparation Method for Proteomics and Metabolomics. Journal of Proteome Research, 19 (7). pp. 2838-2844. ISSN 1535-3893
Abstract
The integration of omics techniques has seen a step change in our understanding of biological systems. However, multiomics has been impaired by mutually exclusive omic separation methods and the destructive nature of the techniques when sample is limited. We describe Simultaneous Trapping (SiTrap), a simple and effective detergent-free method that facilitates direct measurement of the proteome and metabolome in the same sample extract. This “single-pot” multiomics processing is particularly beneficial in cases when sample amounts are limited or are heterogeneous, for example, tissue biopsies. We demonstrate the value of the SiTrap methodology as an essential multiomics tool in a proof-of-principle integrated study of renal cancer tissue biopsy samples. We believe SiTrap has the potential to become an indispensable tool in translational medical research.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society. This is an author produced version of an article published in Journal of Proteome Research. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Multiomics sample preparation, detergent-free sample preparation, SiTrap |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) |
Funding Information: | Funder Grant number BBSRC (Biotechnology & Biological Sciences Research Council) BB/R013500/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 22 Nov 2019 11:08 |
Last Modified: | 19 Nov 2020 01:38 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acs.jproteome.9b00662 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:153763 |