A novel workflow for three-dimensional analysis of tumour cell migration

Abstract

The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal–amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.

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Item Type:	Article
Authors/Creators:	Ketchen, S Rohwedder, A Knipp, S Esteves, F Struve, N Peckham, M https://orcid.org/0000-0002-3754-2028 Ladbury, JE https://orcid.org/0000-0002-6328-7200 Curd, A https://orcid.org/0000-0002-3949-7523 Short, SC https://orcid.org/0000-0003-4423-7256 Bruning-Richardson, A https://orcid.org/0000-0002-9862-9805
Copyright, Publisher and Additional Information:	© 2020 The Author(s). Published by the Royal Society. All rights reserved. This is an author produced version of a paper published in Interface Focus. Uploaded in accordance with the publisher's self-archiving policy.
Keywords:	3D, tumour spheroid, microscopy, ImageJ, cell migration
Dates:	Published: 6 April 2020 Published (online): 14 February 2020 Accepted: 13 November 2019
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Biological Chemistry (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cell Biology (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cancer and Pathology (LICAP) > Brain Tumour Research (Leeds)
Funding Information:	Funder Grant number MRC (Medical Research Council) MR/K015613/1 PPR Foundation Not Known
Depositing User:	Symplectic Publications
Date Deposited:	19 Nov 2019 11:29
Last Modified:	18 Mar 2020 15:38
Status:	Published
Publisher:	The Royal Society
Identification Number:	10.1098/rsfs.2019.0070
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:153594

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A novel workflow for three-dimensional analysis of tumour cell migration

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