Sharp, P.S., Ameen-Ali, K.E., Boorman, L. et al. (6 more authors) (2020) Neurovascular coupling preserved in a chronic mouse model of Alzheimer’s disease : methodology is critical. Journal of Cerebral Blood Flow and Metabolism, 40 (11). pp. 2289-2303. ISSN 0271-678X
Abstract
Impaired neurovascular coupling has been suggested as an early pathogenic factor in Alzheimer’s disease (AD), which could serve as an early biomarker of cerebral pathology. We have established an anaesthetic regime to allow repeated measurements of neurovascular function over three months in the J20 mouse model of AD (J20-AD) and wild-type (WT) controls. Animals were 9-12 months old at the start of the experiment. Mice were chronically prepared with a cranial window through which optical imaging spectroscopy (OIS) was used to generate functional maps of the cerebral blood volume and saturation changes evoked by whisker stimulation and vascular reactivity challenges. Unexpectedly, the hemodynamic responses were largely preserved in the J20-AD group. This result failed to confirm previous investigations using the J20-AD model. However, a final acute electrophysiology and OIS experiment was performed to measure both neural and hemodynamic responses concurrently. In this experiment, previously reported deficits in neurovascular coupling in the J20-AD model were observed. This suggests that J20-AD mice may be more susceptible to the physiologically stressing conditions of an acute experimental procedure compared to WT animals. These results therefore highlight the importance of experimental procedure when determining the characteristics of animal models of human disease.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2019 The Authors. This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
Keywords: | Barrel Cortex; Electrophysiology; Optical Imaging; Blood Flow; J20 |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Psychology (Sheffield) |
Funding Information: | Funder Grant number Medical Research Council MR/M013553/1 Alzheimer's Research UK ARUK-IRG2014-10 Wellcome Trust (The) 105586/Z/14/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Nov 2019 11:51 |
Last Modified: | 30 Apr 2021 12:56 |
Status: | Published |
Publisher: | SAGE Publications |
Refereed: | Yes |
Identification Number: | 10.1177/0271678X19890830 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:153114 |