Camus, Stéphane M, Camus, Marine D, Figueras-Novoa, Carmen et al. (10 more authors) (2020) CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. Journal of Cell Biology. e201812135. ISSN 0021-9525
Abstract
Glucose transporter 4 (GLUT4) is sequestered inside muscle and fat and then released by vesicle traffic to the cell surface in response to postprandial insulin for blood glucose clearance. Here, we map the biogenesis of this GLUT4 traffic pathway in humans, which involves clathrin isoform CHC22. We observe that GLUT4 transits through the early secretory pathway more slowly than the constitutively secreted GLUT1 transporter and localize CHC22 to the ER-to-Golgi intermediate compartment (ERGIC). CHC22 functions in transport from the ERGIC, as demonstrated by an essential role in forming the replication vacuole of Legionella pneumophila bacteria, which requires ERGIC-derived membrane. CHC22 complexes with ERGIC tether p115, GLUT4, and sortilin, and downregulation of either p115 or CHC22, but not GM130 or sortilin, abrogates insulin-responsive GLUT4 release. This indicates that CHC22 traffic initiates human GLUT4 sequestration from the ERGIC and defines a role for CHC22 in addition to retrograde sorting of GLUT4 after endocytic recapture, enhancing pathways for GLUT4 sequestration in humans relative to mice, which lack CHC22.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Keywords: | Adaptor Proteins, Vesicular Transport/metabolism,Biosynthetic Pathways,Cell Membrane/metabolism,Clathrin/metabolism,Clathrin Heavy Chains/metabolism,Endoplasmic Reticulum/metabolism,Glucose Transporter Type 4/metabolism,Golgi Apparatus/metabolism,HeLa Cells,Humans,Protein Transport,Rho Guanine Nucleotide Exchange Factors/metabolism,Vesicular Transport Proteins/metabolism |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 01 Nov 2019 10:30 |
Last Modified: | 16 Oct 2024 16:09 |
Published Version: | https://doi.org/10.1083/jcb.201812135 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.201812135 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:152961 |
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