Battaglia, G., LoPresti, C., Massignani, M. et al. (8 more authors) (2011) Wet nanoscale imaging and testing of polymersomes. Small, 7 (14). pp. 2010-2015. ISSN 1613-6810
Abstract
A facile methodology for the immobilization of polymersomes is demonstrated using ad‐hoc synthesized, biotinylated copolymers or commercially available biotinylated phospholipids on streptavidin‐decorated substrates. This allows the imaging of polymersomes using high‐resolution techniques such as stimulated emission depletion microscopy and atomic force microscopy. This latter allows the gathering of other important functional parameters such as mechanical properties and surface topology, enabling characterization of these water‐borne nanoparticles in wet conditions and hence in their natural environment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of a paper subsequently published in Small. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | polymersomes; atomic force microscopy; STED microscopy; biotin/avidin complexes; force mapping microscopy |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > Department of Physics and Astronomy (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Nov 2019 16:17 |
Last Modified: | 14 Nov 2019 16:17 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/smll.201100511 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:152449 |