Funston, Scott J, Tsaousi, Konstantina, Rudden, Michelle orcid.org/0000-0001-9617-087X et al. (4 more authors) (2016) Characterising rhamnolipid production in Burkholderia thailandensis E264, a non-pathogenic producer. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. pp. 7945-7956. ISSN 0175-7598
Abstract
Burkholderia thailandensis E264 is a rhamnolipid (RL)-producing gram-negative bacterium first isolated from the soils and stagnant waters of central and north-eastern Thailand. Growth of B. thailandensis E264 under two different incubation temperatures (25 and 30 °C) resulted in a significantly higher dry cell biomass production at 30 °C (7.71 g/l) than at 25 °C (4.75 g/l) after 264 h; however, incubation at the lower temperature resulted in consistently higher concentration of RL production throughout the growth period. After 264 h, the concentration of crude RL extract for the 25 °C culture was 2.79 g/l compared to 1.99 g/l for the 30 °C culture. Overall RL production concentration after 264 h was 0.258 g/g dry cell biomass (DCB) for the 30 °C culture compared to 0.587 g/g DCB for the 25 °C culture. Real-time PCR (qPCR) was also used to analyse expression of the RL biosynthesis genes throughout the incubation period at 25 °C showing that the expression of the rhlA, rhlB and rhlC genes is continuous. During the log and early stationary phases of growth, expression levels remain low and are increased upon entry to the late stationary phase. B. thailandensis E264 produces mostly di-RLs and the Di-RL C14-C14 in most abundance (41.88 %). Fermentations were also carried out in small-scale bioreactors (4 l working volume) under controlled conditions, and results showed that RL production was maintained. Our findings show that B. thailandensis E264 has excellent potential for industrial scale RL production.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | Burkholderia/genetics,Gene Expression Profiling,Glycolipids/metabolism,Metabolic Networks and Pathways,Real-Time Polymerase Chain Reaction,Temperature |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 30 Sep 2019 13:00 |
Last Modified: | 15 Dec 2024 00:07 |
Published Version: | https://doi.org/10.1007/s00253-016-7564-y |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1007/s00253-016-7564-y |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:151494 |
Download
Filename: Funston2016_Article_CharacterisingRhamnolipidProdu.pdf
Description: Funston2016_Article_CharacterisingRhamnolipidProdu
Licence: CC-BY 2.5