Byrne, MJ orcid.org/0000-0001-8111-4605, Steele, JFC, Hesketh, EL et al. (4 more authors) (2019) Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles. Structure, 27 (12). 1761-1770.e3. ISSN 0969-2126
Abstract
The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/L021250/1 Wellcome Trust 108466/Z/15/Z BBSRC BB/R00160X/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Sep 2019 10:53 |
Last Modified: | 25 Jun 2023 21:59 |
Status: | Published |
Publisher: | Cell Press |
Identification Number: | 10.1016/j.str.2019.09.010 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:151255 |