Foyer, CH orcid.org/0000-0001-5989-6989, Pellny, TK, Locato, V et al. (2 more authors) (2019) Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione, and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures. In: Hancock, JT and Conway, ME, (eds.) Redox-Mediated Signal Transduction. Methods in Molecular Biology, 1990 . Springer US , New York, NY, USA , pp. 165-181. ISBN 978-1-4939-9461-8
Abstract
Reactive oxygen species (ROS) and low molecular weight antioxidants, such as glutathione and ascorbate, are powerful signalling molecules that participate in the control of plant growth and development, and modulate progression through the mitotic cell cycle. Enhanced ROS accumulation or low levels of ascorbate or glutathione cause the cell cycle to arrest and halt progression especially through the G1 checkpoint. Plant cell suspension cultures have proved to be particularly useful tools for the study of cell cycle regulation. Here we provide effective and accurate methods for the measurement of changes in the cellular ascorbate and glutathione pools and the activities of related enzymes such poly(ADP-ribose)polymerase (PARP) during mitosis and cell expansion, particularly in cell suspension cultures. These methods can be used in studies seeking to improve current understanding of the roles of redox controls on cell division and cell expansion.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2019, Springer Science+Business Media, LLC, part of Springer Nature. This is an author produced version of a chapter published in Redox-Mediated Signal Transduction. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Antioxidants; Plant cell suspension cultures; Cell cycle; Cellular redox state; Oxidative stress; Mitochondria; Cell death; Poly(ADPribose) polymerase |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Nov 2019 15:57 |
Last Modified: | 31 May 2020 00:38 |
Status: | Published |
Publisher: | Springer US |
Series Name: | Methods in Molecular Biology |
Identification Number: | 10.1007/978-1-4939-9463-2_14 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:150698 |