Miller, Lisa Margaret orcid.org/0000-0003-3667-3840, Silver, Callum David, Herman, Reyme et al. (4 more authors) (2019) Surface-Bound Antibiotic for the Detection of β-Lactamases. ACS applied materials & interfaces. ISSN 1944-8252
Abstract
Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial resistance is to b-lactam antibiotics (e.g. penicillin). This resistance is often caused by b-lactamases, which hydrolyze b-lactam drugs, rendering them ineffective. Current methods for detecting these enzymes either require time- consuming growth assays or antibiotic mimics such as nitrocefin. Here, we report the development of a surface-bound, clinically- relevant, b-lactam drug that can be used to detect b-lactamases. Furthermore, we demonstrate the use of these functionalized surfaces to selectively detect b-lactamases in complex biological media, such as urine.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Electronic Engineering (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number EPSRC EP/P02324X/1 EPSRC EP/L024829/1 |
Depositing User: | Pure (York) |
Date Deposited: | 03 Sep 2019 15:30 |
Last Modified: | 16 Dec 2024 00:10 |
Published Version: | https://doi.org/10.1021/acsami.9b05793 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1021/acsami.9b05793 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:150274 |