Scarff, CA orcid.org/0000-0001-6168-0060, Ashcroft, AE orcid.org/0000-0002-1676-123X and Radford, SE orcid.org/0000-0002-3079-8039 (2016) Characterization of Amyloid Oligomers by Electrospray Ionization-Ion Mobility Spectrometry-Mass Spectrometry (ESI-IMS-MS). In: Eliezer, D, (ed.) Protein Amyloid Aggregation: methods and protocols. Methods in Molecular Biology, 1345 . Springer (Humana Press) , New York, N.Y., U.S.A. , pp. 115-132. ISBN 978-1-4939-2977-1
Abstract
Soluble oligomers formed during the self-assembly of amyloidogenic peptide and protein species are generally thought to be highly toxic. Consequently, thorough characterization of these species is of much interest in the quest for effective therapeutics and for an enhanced understanding of amyloid fibrillation pathways. The structural characterization of oligomeric species, however, is challenging as they are often transiently and lowly populated, and highly heterogeneous. Electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) is a powerful technique which is able to detect individual ion species populated within a complex heterogeneous mixture and characterize them in terms of shape, stoichiometry, ligand binding capability, and relative stability. Herein, we describe the use of ESI-IMS-MS to characterize the size and shape of oligomers of beta-2-microglobulin through use of data calibration and the derivation of models. This enables information about the range of oligomeric species populated en route to amyloid formation and the mode of oligomer growth to be obtained.
Metadata
Item Type: | Book Section |
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Copyright, Publisher and Additional Information: | (c) 2016, Springer Science+Business Media New York. This is an author produced version of a chapter published in Protein Amyloid Aggregation: methods and protocols. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Protein aggregation; Amyloid; Oligomerization; Native mass spectrometry; Ion mobility spectrometry-mass spectrometry |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Animal Cell Biology (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Biomolecular Mass Spectroscopy (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Aug 2019 12:51 |
Last Modified: | 12 Aug 2019 12:51 |
Status: | Published |
Publisher: | Springer (Humana Press) |
Series Name: | Methods in Molecular Biology |
Identification Number: | 10.1007/978-1-4939-2978-8_8 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149599 |