Marsian, J, Hurdiss, DL, Ranson, NA orcid.org/0000-0002-3640-5275 et al. (4 more authors) (2019) Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease. Frontiers in Plant Science, 10. 880. ISSN 1664-462X
Abstract
Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, Marsian, Hurdiss, Ranson, Ritala, Paley, Cano and Lomonossof. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: | Atlantic cod nervous necrosis virus; virus-like particles; transient expression; sea bass; protective immunity |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Aug 2019 10:44 |
Last Modified: | 06 Aug 2019 10:44 |
Status: | Published |
Publisher: | Frontiers Media |
Identification Number: | 10.3389/fpls.2019.00880 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149337 |