Spurgeon, BEJ and Naseem, KM (2020) Platelet Flow Cytometry: Instrument Setup, Controls, and Panel Performance. Cytometry Part B: Clinical Cytometry, 98 (1). pp. 19-27. ISSN 1552-4949
Abstract
Platelet flow cytometry is widely used in cardiovascular medicine as the platelet surface is rich in clinical biomarkers. Surface profiling is critical in disease management, but current assays can abet clinical errors as they are suboptimal and prone to bias. Accordingly, the technical and analytical advances that can be used to create high quality assays with minimal error and maximal sensitivity were reviewed. Specifically, the best practices for instrument setup, quality control, panel design, titration, gating, and compensation were described. Adherence to these practices will enhance the validity and reliability of platelet flow cytometry in clinical/research settings.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | blood platelets; cardiovascular diseases; flow cytometry; immunophenotyping; platelet function tests |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) |
Funding Information: | Funder Grant number Heart Research UK RG2659/17/19 |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Aug 2019 10:11 |
Last Modified: | 30 Jan 2020 16:29 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/cyto.b.21774 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149331 |