Mahon, CS, Wildsmith, GC, Haksar, D et al. (5 more authors) (2019) A ‘catch-and-release’ receptor for the cholera toxin. Faraday Discussions, 219. pp. 112-127. ISSN 1359-6640
Abstract
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, The Royal Society of Chemistry. This is an author produced version of a paper published in Faraday Discussions. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Aug 2019 16:16 |
Last Modified: | 21 Mar 2020 01:38 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/c9fd00017h |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149299 |