Saha, S, Yang, XB orcid.org/0000-0002-0144-2826, Wijayathunga, N et al. (4 more authors) (2019) A biomimetic self-assembling peptide promotes bone regeneration in vivo: A rat cranial defect study. Bone, 127. pp. 602-611. ISSN 8756-3282
Abstract
Rationally designed, pH sensitive self-assembling β-peptides (SAPs) which are capable of reversibly switching between fluid and gel phases in response to environmental triggers are potentially useful injectable scaffolds for skeletal tissue engineering applications. SAP P11-4 (CH3COQQRFEWEFEQQNH2) has been shown to nucleate hydroxyapatite mineral de novo and has been used in dental enamel regeneration. We hypothesised that addition of mesenchymal stromal cells (MSCs) would enhance the in vivo effects of P11-4 in promoting skeletal tissue repair. Cranial defects were created in athymic rats and filled with either Bio-Oss® (anorganic bone chips) or P11-4 ± human dental pulp stromal cells (HDPSCs). Unfilled defects served as controls. After 4 weeks, only those defects filled with P11-4 alone showed significantly increased bone regeneration (almost complete healing), compared to unfilled control defects, as judged using quantitative micro-CT, histology and immunohistochemistry. In silico modelling indicated that fibril formation may be essential for any mineral nucleation activity. Taken together, these data suggest that self-assembling peptides are a suitable scaffold for regeneration of bone tissue in a one step, cell-free therapeutic approach.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Crown Copyright © 2019 Published by Elsevier Inc. All rights reserved. This is an author produced version of an article Bone. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Self-assembly; Hydrogels; Bone regeneration; Mesenchymal stromal cells; Calvaria |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 088908/Z/09/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 02 Aug 2019 09:14 |
Last Modified: | 24 Jul 2020 00:39 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bone.2019.06.020 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:149245 |