Moroz, Olga V., Blagova, Elena, Lebedev, Andrey A. et al. (5 more authors) (2017) The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium. Acta crystallographica. Section D, Structural biology. pp. 32-44. ISSN 2059-7983
Abstract
Bacterial phosphoinositide-specific phospholipases C (PI-PLCs) are the smallest members of the PI-PLC family, which includes much larger mammalian enzymes responsible for signal transduction as well as enzymes from protozoan parasites, yeast and plants. Eukaryotic PI-PLCs have calcium in the active site, but this is absent in the known structures of Gram-positive bacteria, where its role is instead played by arginine. In addition to their use in a number of industrial applications, the bacterial enzymes attract special interest because they can serve as convenient models of the catalytic domains of eukaryotic enzymes for in vitro activity studies. Here, the structure of a PI-PLC from Pseudomonas sp. 62186 is reported, the first from a Gram-negative bacterium and the first of a native bacterial PI-PLC with calcium present in the active site. Solution of the structure posed particular problems owing to the low sequence identity of available homologous structures. Its dependence on calcium for catalysis makes this enzyme a better model for studies of the mammalian PI-PLCs than the previously used calcium-independent bacterial PI-PLCs.The structure of the calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186 is described, the first from a Gram-negative bacterium. Structure solution from a low-sequence-identity homology model is described in detail.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | ©2017 International Union of Crystallography. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Keywords: | calcium-dependent,Gram-negative bacterium,phosphoinositol-specific,phospholipase C,Pseudomonas sp. 62186,X-ray structure |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 25 Jun 2019 10:20 |
Last Modified: | 23 Oct 2024 23:58 |
Published Version: | https://doi.org/10.1107/S2059798316019616 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1107/S2059798316019616 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:147783 |