First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study

Abstract

The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.

Metadata

Item Type:	Article
Authors/Creators:	Iacobucci, C Piotrowski, C Aebersold, R Amaral, BC Andrews, P Bernfur, K Borchers, C Brodie, NI Bruce, JE Cao, Y Chaignepain, S Chavez, JD Claverol, S Cox, J Davis, T Degliesposti, G Dong, M-Q Edinger, N Emanuelsson, C Gay, M Götze, M Gomes-Neto, F Gozzo, FC Gutierrez, C Haupt, C Heck, AJR Herzog, F Huang, L Hoopmann, MR Kalisman, N Klykov, O Kukačka, Z Liu, F MacCoss, MJ Mechtler, K Mesika, R Moritz, RL Nagaraj, N Nesati, V Neves-Ferreira, AGC Ninnis, R Novák, P O'Reilly, FJ Pelzing, M Petrotchenko, E Piersimoni, L Plasencia, M Pukala, T Rand, KD Rappsilber, J Reichmann, D Sailer, C Sarnowski, CP Scheltema, RA Schmidt, C Schriemer, DC Shi, Y Skehel, JM Slavin, M Sobott, F https://orcid.org/0000-0001-9029-1865 Solis-Mezarino, V Stephanowitz, H Stengel, F Stieger, CE Trabjerg, E Trnka, M Vilaseca, M Viner, R Xiang, Y Yilmaz, S Zelter, A Ziemianowicz, D Leitner, A Sinz, A
Copyright, Publisher and Additional Information:	(c) 2019, American Chemical Society. This is an open access article published under an ACS AuthorChoice License [https://pubs.acs.org/page/policy/authorchoice_termsofuse.html], which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Dates:	Accepted: 2 May 2019 Published (online): 22 May 2019 Published: 4 June 2019
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds)
Depositing User:	Symplectic Publications
Date Deposited:	26 Jun 2019 16:26
Last Modified:	26 Jun 2019 16:26
Status:	Published
Publisher:	American Chemical Society
Identification Number:	10.1021/acs.analchem.9b00658
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:147746

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First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study

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