Sandlund, J and Wilcox, MH orcid.org/0000-0002-4565-2868 (2019) Ultrasensitive Detection of Clostridium difficile Toxins Reveals Suboptimal Accuracy by Toxin-Gene Cycle-Thresholds for Toxin Predictions. Journal of clinical microbiology, 57 (6). e01885-18. ISSN 0095-1137
Abstract
The use of nucleic acid amplification tests (NAATs) for the diagnosis of Clostridium (Clostridioides) difficile infection (CDI) leads to overdiagnosis. To improve the clinical specificity of NAATs, there has been a recent interest in using toxin-gene cycle-thresholds (CT) to predict the presence and absence of toxins. Although there is an association between CT values and fecal-toxin concentrations, the predictive accuracy of the former is suboptimal for use in clinical practice. Ultrasensitive toxin immunoassays to quantify free toxins in stool offer a novel option for high-sensitivity fecal-toxin detection, rather than using surrogate markers for prediction.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Society for Microbiology. This is an author produced version of a paper published in Journal of clinical microbiology. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 May 2019 14:05 |
Last Modified: | 03 Sep 2019 00:43 |
Status: | Published |
Publisher: | American Society for Microbiology |
Identification Number: | 10.1128/jcm.01885-18 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:146331 |