Damé-Teixeira, N, Deng, D and Do, T orcid.org/0000-0002-5668-2181 (2019) Streptococcus mutans transcriptome in the presence of sodium fluoride and sucrose. Archives of Oral Biology, 102. pp. 186-192. ISSN 0003-9969
Abstract
Objective:
Considering the diverse mechanisms by which fluoride could affect oral bacteria, this study evaluated the effect of sodium fluoride onStreptococcus mutans transcriptome in the presence of sucrose.
Methods:
S. mutans UA159 was cultured in 3 different types of media: medium control[TY], sucrose control [TY_S], and sodium fluoride sucrose test[TY_S_NaF]. Triplicates of each group were sampled at exponential phase 3 h after inoculation, total RNA was isolated, mRNA enriched and cDNA paired-end sequenced (Illumina Hi-Seq2500).
Results:
Genes related toS. mutans adhesion(gtfB and gtfC), acidogenicity and sugar transport were up-regulated in the presence of sucrose(TY_S) and sucrose/fluoride(TY_S_NaF), whereas gene dltA, D-alanine-activating enzyme, which is related to regulation of non-PTS sugar internalization was down-regulated. Up-regulation of the scrA gene and the PTS fructose-and mannose system, as well as functions such as those involved in stress and defence responses and peptidases; and down-regulation of lacACDG and pyruvate formate-lyase were observed in the TY_S_NaF group, as compared to TY_S group.
Conclusions:
The presence of NaF has decreased the overall gene expression level inS. mutans. However, its major effect seems to be the inducing of expression of genes involved in some PEP:PTS systems and other metabolic transporters which imply specific cellular internalisation of sugars.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier Ltd. All rights reserved. This is an author produced version of an article published in the Archives of Oral Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Streptococcus mutans; Gene expression; Sodium fluoride; Sucrose |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 May 2019 13:56 |
Last Modified: | 01 May 2020 00:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.archoralbio.2019.04.020 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145531 |