Twarock, R and Stockley, PG orcid.org/0000-0002-1360-2751 (2019) RNA-Mediated Virus Assembly: Mechanisms and Consequences for Viral Evolution and Therapy. Annual Review of Biophysics, 48 (1). pp. 495-514. ISSN 1936-122X
Abstract
Viruses, entities composed of nucleic acids, proteins, and in some cases lipids lack the ability to replicate outside their target cells. Their components self-assemble at the nanoscale with exquisite precision-a key to their biological success in infection. Recent advances in structure determination and the development of biophysical tools such as single-molecule spectroscopy and noncovalent mass spectrometry allow unprecedented access to the detailed assembly mechanisms of simple virions. Coupling these techniques with mathematical modeling and bioinformatics has uncovered a previously unsuspected role for genomic RNA in regulating formation of viral capsids, revealing multiple, dispersed RNA sequence/structure motifs [packaging signals (PSs)] that bind cognate coat proteins cooperatively. The PS ensemble controls assembly efficiency and accounts for the packaging specificity seen in vivo. The precise modes of action of the PSs vary between viral families, but this common principle applies across many viral families, including major human pathogens. These insights open up the opportunity to block or repurpose PS function in assembly for both novel antiviral therapy and gene/drug/vaccine applications. Expected final online publication date for the Annual Review of Biophysics Volume 48 is May 3, 2019. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | virus assembly, RNA packaging signals, genome organization, mathematical modeling, single-molecule FCS, normal mode analysis, Gillespie algorithms, Hamiltonian paths |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Biological Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 May 2019 10:42 |
Last Modified: | 18 Jul 2019 14:45 |
Status: | Published |
Publisher: | Annual Reviews |
Identification Number: | 10.1146/annurev-biophys-052118-115611 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145524 |