Blakeman, J.T., Morales-García, A.L. orcid.org/0000-0002-4451-3225, Mukherjee, J. et al. (4 more authors) (2019) Extracellular DNA provides structural integrity to a Micrococcus luteus biofilm. Langmuir, 35 (19). pp. 6468-6475. ISSN 0743-7463
Abstract
Force spectroscopy was used to show that extracellular DNA (eDNA) has a pre-eminent structural role in a biofilm. The adhesive behavior of extracellular polymeric substances to poly(ethylene terephthalate), a model hydrophobic surface, was measured in response to their degradation by hydrolytic enzymes known for their biofilm dispersion potential: DNaseI, protease, cellulase, and mannanase. Only treatment with DNaseI significantly decreased the adhesive force of the model bacterium Micrococcus luteus with the surface, and furthermore this treatment almost completely eliminated any components of the biofilm maintaining the adhesion, establishing a key structural role for eDNA.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society. This is an author-produced version of a paper subsequently published in Langmuir. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Physics and Astronomy (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Apr 2019 11:49 |
Last Modified: | 23 Nov 2021 15:21 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acs.langmuir.9b00297 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145192 |