Mahon, Clare Sarah, Wildsmith, Gemma C., Haksar, Diksha et al. (5 more authors) (2019) A ‘catch-and-release’ receptor for the cholera toxin. FARADAY DISCUSSIONS. ISSN 1364-5498
Abstract
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2019. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 17 Apr 2019 14:50 |
Last Modified: | 14 Mar 2025 00:08 |
Published Version: | https://doi.org/10.1039/C9FD00017H |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1039/C9FD00017H |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145163 |
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