Pokhrel, B, Chen, Y and Biro, JJ (2019) CFP‐1 interacts with HDAC1/2 complexes in C. elegans development. The FEBS Journal, 286 (13). pp. 2490-2504. ISSN 1742-464X
Abstract
CXXC finger binding protein 1 (CFP‐1) is an evolutionarily conserved protein that binds to non‐methylated CpG‐rich promoters in mammals and Caenorhabditis elegans. This conserved epigenetic regulator is part of the COMPASS complex that contains the H3K4me3 methyltransferase SET1 in mammals and SET‐2 in C. elegans. Previous studies have indicated the importance of CFP1 in embryonic stem cell differentiation and cell fate specification. However, neither the function nor the mechanism of action of CFP1 is well understood at the organismal level. Here, we have used cfp‐1(tm6369) and set‐2(bn129) C. elegans mutants to investigate the function of CFP‐1 in gene induction and development. We have characterised C. elegansCOMPASS mutants cfp‐1(tm6369) and set‐2(bn129) and found that both cfp‐1 and set‐2 play an important role in the regulation of fertility and development of the organism. Furthermore, we found that both cfp‐1 and set‐2 are required for H3K4 trimethylation and play a repressive role in the expression of heat shock and salt‐inducible genes. Interestingly, we found that cfp‐1 but not set‐2 genetically interacts with histone deacetylase (HDAC1/2) complexes to regulate fertility, suggesting a function of CFP‐1 outside of the COMPASS complex. Additionally, we found that cfp‐1 and set‐2 independently regulate fertility and development of the organism. Our results suggest that CFP‐1 genetically interacts with HDAC1/2 complexes to regulate fertility, independent of its function within the COMPASS complex. We propose that CFP‐1 could cooperate with the COMPASS complex and/or HDAC1/2 in a context‐dependent manner.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, Federation of European Biochemical Societies. This is the peer reviewed version of the following article: 'Pokhrel, B, Chen, Y and Biro, JJ (2019) CFP-1 interacts with HDAC1/2 complexes in C. elegans development. The FEBS Journal,' which has been published in final form at [https://doi.org/10.1111/febs.14833]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | cfp‐1; H3K4me3; Set1/COMPASS complex and HDACs; set‐2 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Apr 2019 15:16 |
Last Modified: | 03 Apr 2020 00:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/febs.14833 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:145018 |