Balzano, Marielle, De Grandis, Maria, Vu Manh, Thien-Phong et al. (19 more authors) (2019) Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell reports. 3257–3271. ISSN 2211-1247
Abstract
In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1−/− mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 The Author(s). |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Centre for Immunology and Infection (CII) (York) |
Depositing User: | Pure (York) |
Date Deposited: | 27 Mar 2019 09:30 |
Last Modified: | 16 Oct 2024 15:34 |
Published Version: | https://doi.org/10.1016/j.celrep.2019.02.065 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.celrep.2019.02.065 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:144155 |
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Description: Nidogen-1 Contributes to the Interaction NetworkInvolved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche
Licence: CC-BY-NC-ND 2.5