Oochi, A, Hajny, J, Fukui, K et al. (11 more authors) (2019) Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization. Plant Physiology, 180 (2). pp. 1152-1165. ISSN 0032-0889
Abstract
Polar auxin transport plays a pivotal role in plant growth and development. PIN-FORMED (PIN) auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis (Arabidopsis thaliana). PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) Copyright 2019 by the American Society of Plant Biologists. This is an author produced version of a paper published in Plant Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Mar 2019 17:27 |
Last Modified: | 01 Apr 2020 00:38 |
Status: | Published |
Publisher: | American Society of Plant Biologists |
Identification Number: | 10.1104/pp.19.00201 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:144101 |