Robinson, Alastair David, Richardson, Peter Michael orcid.org/0000-0002-6631-2459 and Halse, Meghan Eileen orcid.org/0000-0002-3605-5551 (2019) Hyperpolarised 1H-13C benchtop NMR spectroscopy. Applied Sciences. 1173. ISSN 2076-3417
Abstract
Benchtop NMR spectrometers with sub-ppm spectral resolution have opened up new opportunities for performing NMR outside of the standard laboratory environment. However, the relatively weak magnetic fields of these devices (1 - 2 T) results in low sensitivity and significant peak overlap in 1H NMR spectra. Here we use hyperpolarised 13C{1H} NMR to overcome these challenges. Specifically, we demonstrate the use of the signal amplification by reversible exchange (SABRE) parahydrogen-based hyperpolarisation technique to enhance the sensitivity of natural abundance 1D and 2D 13C{1H} benchtop NMR spectra. We compare two detection methods for SABRE-enhanced 13C NMR and observe an optimal 13C{1H} signal-to-noise ratio (SNR) for a refocused INEPT approach, where hyperpolarisation is transferred from 1H to 13C. In addition, we exemplify SABRE-enhanced 2D 13C benchtop NMR through the acquisition of a 2D HETCOR spectrum of 260 mM of 4-methylpyridine at natural isotopic abundance in a total experiment time of 69 mins. In theory, signal averaging for over 300 days would be required to achieve a comparable SNR for a thermally polarised benchtop NMR spectrum acquired of a sample of the same concentration at natural abundance
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 by the authors |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number EPSRC EP/R028745/1 EPSRC EP/M020983/1 EPSRC EP/R51181X/1 |
Depositing User: | Pure (York) |
Date Deposited: | 15 Mar 2019 14:30 |
Last Modified: | 07 Feb 2025 00:24 |
Published Version: | https://doi.org/10.3390/app9061173 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3390/app9061173 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:143732 |
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