Walko, M, Hewitt, E orcid.org/0000-0002-6238-6303, Radford, SE orcid.org/0000-0001-9668-6366 et al. (1 more author) (2019) Design and Synthesis of Cysteine-Specific Labels for Photo-Crosslinking Studies. RSC Advances, 9 (14). pp. 7610-7614. ISSN 2046-2069
Abstract
Chemical cross-linking mass-spectrometry (XL-MS) represents a powerful methodology to map ligand/biomacromolecule interactions, particularly where conventional methods such as X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy or cryo-electron microscopy (EM) are not feasible. In this manuscript, we describe the design and synthesis of two new photo-crosslinking reagents that can be used to specifically label free thiols through either maleimido or methanethiosulfonate groups and facilitate PXL-MS workflows. Both crosslinkers are based on light sensitive diazirines – precursors of highly reactive carbenes which offer additional advantages over alternative crosslinking groups such as benzophenones and aryl nitrenes given the controlled rapid and more indiscriminate reactivity.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019,The Royal Society of Chemistry. This is an open access article under the terms of the Creative Commons Attribution License 3.0 Unported (CC BY 3.0). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Funding Information: | Funder Grant number EPSRC EP/N035267/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Feb 2019 12:51 |
Last Modified: | 25 Jun 2023 21:44 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/c8ra10436k |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:143072 |