Dikici, S. orcid.org/0000-0001-9933-5254, Mangır, N., Claeyssens, F. et al. (2 more authors) (2019) Exploration of 2-deoxy-D-ribose and 17β-Estradiol as alternatives to exogenous VEGF to promote angiogenesis in tissue-engineered constructs. Regenerative Medicine, 14 (3). pp. 179-197. ISSN 1746-0751
Abstract
Aim: In this study, we explored the angiogenic potential and proangiogenic concentration ranges of 2-deoxy-D-ribose (2dDR) and 17β-Estradiol (E2) in comparison with VEGF. The 2dDR and E2 were then loaded into tissue engineering (TE) scaffolds to investigate their proangiogenic potential when released from fibers. Materials & methods:Ex ovo chick chorioallantoic membrane (CAM) assay was used to evaluate angiogenic activity of 2dDR and E2. Both factors were then introduced into scaffolds via electrospinning to assess their angiogenic potential when released from fibers. Results: Both factors were approximately 80% as potent as VEGF and showed a dose-dependent angiogenic response. The sustained release of both agents from the scaffolds stimulated neovascularization over 7 days in the chorioallantoic membrane assay. Conclusion: We conclude that both 2dDR and E2 provide attractive alternatives to VEGF for the functionalization of tissue engineering scaffolds to promote angiogenesis in vivo.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Future Medicine Ltd. This is an author produced version of a paper subsequently published in Regenerative Medicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | 2-deoxy-D-ribose; angiogenesis; chick chorioallantoic membrane assay; estradiol; VEGF |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 27 Feb 2019 09:58 |
Last Modified: | 18 Nov 2021 13:57 |
Status: | Published |
Publisher: | Future Medicine |
Refereed: | Yes |
Identification Number: | 10.2217/rme-2018-0068 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142977 |