Davidson, A.J., Millard, T.H., Evans, I.R. orcid.org/0000-0002-3485-4456 et al. (1 more author) (2019) Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxis. Journal of Cell Science, 132 (5). jcs224618. ISSN 0021-9533
Abstract
The actin cytoskeleton is the engine that powers the inflammatory chemotaxis of immune cells to sites of tissue damage or infection. Here we combine genetics with live, in vivo imaging to investigate how cytoskeletal rearrangements drive macrophage recruitment to wounds in Drosophila We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin. In contrast, the formin Dia generates rare, probing filopods for specialised functions that are not required for migration.Ena's role in lamellipodial bundling is so fundamental that its over-expression increases bundling even in the absence of Fascin by marshalling the remaining cross-linking proteins to compensate. This reorganisation of the lamellipod generates cytoskeletal struts that push against the membrane to drive leading edge advancement and boost cell speed. Thus, Ena-mediated remodeling extracts the most from the cytoskeleton to power robust macrophage chemotaxis during their inflammatory recruitment to wounds.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
Keywords: | Actin; Drosophila; Ena; Hemocyte; Macrophage; Migration |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Mar 2019 15:46 |
Last Modified: | 11 Mar 2019 11:59 |
Published Version: | https://doi.org/10.1242/jcs.224618 |
Status: | Published |
Publisher: | Company of Biologists |
Refereed: | Yes |
Identification Number: | 10.1242/jcs.224618 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142788 |