Guo, X, Runavot, J-L, Bourot, S et al. (8 more authors) (2019) Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development. Planta, 249 (5). pp. 1565-1581. ISSN 0032-0935
Abstract
Main conclusion:
Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism.
Cotton fibres are single cell structures that early in development adhere to one another via the cotton fibre middle lamella (CFML) to form a tissue-like structure. The CFML is disassembled around the time of initial secondary wall deposition, leading to fibre detachment. Observations of CFML in the light microscope have suggested that the development of the middle lamella is accompanied by substantial cell-wall metabolism, but it has remained an open question as to which processes mediate adherence and which lead to detachment. The mechanism of adherence and detachment were investigated here using glyco-microarrays probed with monoclonal antibodies, transcript profiling, and observations of fibre auto-digestion. The results suggest that adherence is brought about by cutin dilution, while the presence of relevant enzyme activities and the dynamics of rhamnogalacturonan-I side-chain accumulation and disappearance suggest that both attachment and detachment are accompanied by rhamnogalacturonan-I metabolism.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. This is a post-peer-review, pre-copyedit version of an article published in Planta. The final authenticated version is available online at: https://doi.org/10.1007/s00425-019-03107-4. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Arabinofuranosidase; Cuticle; Post-genital fusion; Rhamnogalacturonan-I; Xyloglucan |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Funding Information: | Funder Grant number EU - European Union 263916 EU - European Union 263916 |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Feb 2019 14:42 |
Last Modified: | 08 Feb 2020 01:38 |
Status: | Published |
Publisher: | Springer Berlin Heidelberg |
Identification Number: | 10.1007/s00425-019-03107-4 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142664 |