Abeln, Felix, Fan, Jiajun orcid.org/0000-0003-3721-5745, Budarin, Vitaliy L. et al. (6 more authors) (2019) Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima. Algal Research. 101411. ISSN 2211-9264
Abstract
Macroalgae (seaweeds) represent an emerging resource for food and the production of commodity and specialty chemicals. In this study, a single-step microwave process was used to depolymerise a range of macroalgae native to the United Kingdom, producing a growth medium suitable for microbial fermentation. The medium contained a range of mono- and polysaccharides as well as macro- and micronutrients that could be metabolised by the oleaginous yeast Metschnikowia pulcherrima. Among twelve macroalgae species, the brown seaweeds exhibited the highest fermentation potential, especially the kelp Saccharina latissima. Applying a portfolio of ten native M. pulcherrima strains, yeast growth kinetics, as well as production of lipids and 2-phenylethanol were examined, with productivity and growth rate being strain dependent. On the 2 L scale, 6.9 g L−1 yeast biomass – a yield of 0.14 g g−1 with respect to the supplied macroalgae – containing 37.2% (w/w) lipid was achieved through utilisation of the proteins, mono- and polysaccharides from S. latissima, with no additional enzymes. In addition, the yeast degraded a range of fermentation inhibitors released upon microwave processing at high temperatures and long holding times. As macroalgae can be cultured to food grade, this system offers a novel, potentially low-cost route to edible microbial oils as well as a renewable feedstock for oleochemicals.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier B.V. All rights reserved. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. |
Keywords: | Macroalgae,Marine biorefinery,Metschnikowia pulcherrima,Microbial lipids,Microwave depolymerisation,Saccharina latissima |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 13 Feb 2019 12:10 |
Last Modified: | 21 Jan 2025 17:37 |
Published Version: | https://doi.org/10.1016/j.algal.2019.101411 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.algal.2019.101411 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142490 |
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Filename: Uncorrected_Seaweed_paper_with_Bath_pagination_ALGAL_101411_cjc.pdf
Description: Author Accepted Manuscript - Seaweed paper with Bath
Licence: CC-BY-NC-ND 2.5