Baker, SR orcid.org/0000-0002-3147-4925, Zabczyk, M, Macrae, FL orcid.org/0000-0002-7092-0422 et al. (3 more authors) (2019) Recurrent venous thromboembolism patients form clots with lower elastic modulus than those with non-recurrent disease. Journal of Thrombosis and Haemostasis, 17 (4). pp. 618-626. ISSN 1538-7933
Abstract
BACKGROUND:Venous thromboembolism (VTE) is associated with a high risk of recurrent events after withdrawal of anticoagulation. OBJECTIVES:The aim of this study was to determine the difference in plasma clot mechanical properties from patients having recurrent (r) versus non-recurrent (nr) VTE. METHODS:We previously developed a system for determining clot mechanical properties using an in-house magnetic tweezers system. This system was used to determine the mechanical properties of clots made from plasma of 11 patients with rVTE and 33 with nrVTE. Plasma was mixed with micrometre-sized beads followed by the addition of thrombin and calcium to induce clotting, then placed in small capillary tubes and allowed to clot overnight. Bead displacements upon manipulation with magnetic forces were analysed to determine clot elastic and viscous moduli. Fibrin clot structure was analysed by turbidimetry and confocal microscopy. FXIII was measured by pentylamine incorporation into fibrin. RESULTS:Clots from rVTE patients showed nearly two-fold less elastic and less viscous moduli than clots from nrVTE patients, regardless of male sex, unprovoked events, family history of VTE, fibrinogen concentration or BMI. No differences were observed in clot structure, fibrinolysis rates or FXIII levels. CONCLUSION:Using magnetic tweezers for the first time in patient samples, we found that plasma clots from rVTE patients demonstrated reduced elastic modulus and reduced viscous modulus when compared with nrVTE patients. These data indicate a possible role for fibrin clot visco-elastic properties in determining VTE recurrence.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Ariens et. al. © 2019 International Society on Thrombosis and Haemostasis. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | fibrin; mechanics; recurrent event; rheology; venous thromboembolism |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) |
Funding Information: | Funder Grant number British Heart Foundation RG/13/3/30104 |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Feb 2019 16:41 |
Last Modified: | 09 May 2019 15:27 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/jth.14402 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:142400 |
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