Wang, M, Gu, Z, Wang, R et al. (4 more authors) (2019) Plant primary metabolism regulated by nitrogen contributes to plant-pathogen interactions. Plant and Cell Physiology, 60 (2). pp. 329-342. ISSN 0032-0781
Abstract
Nitrogen contributes to plant defense responses by the regulation of plant primary metabolism during plant–pathogen interactions. Based on biochemical, physiological, bioinformatic and transcriptome approaches, we investigated how different nitrogen forms (ammonium vs. nitrate) regulate the physiological response of cucumber (Cucumis sativus) to Fusarium oxysporum f. sp. cucumerinum (FOC) infection. The metabolic profile revealed that nitrate-grown plants accumulated more organic acids, while ammonium-grown plants accumulated more amino acids; FOC infection significantly increased levels of both amino acids and organic acids in the roots of ammonium-grown plants. Transcriptome analysis showed that genes related to carbon metabolism were mostly up-regulated in plants grown with nitrate, whereas in ammonium-grown plants the up-regulated genes were mostly those that were related to primary nitrogen metabolism. Root FOC colonization and disease incidence were positively correlated with levels of root amino acids and negatively correlated with levels of root organic acids. In conclusion, organic acid metabolism and expression of related genes increased under nitrate, whereas ammonium increased the level of amino acids and expression of related genes; these altered levels of organic acids and amino acids resulted in different tolerances to FOC infection depending on the nitrogen forms supplied.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2018. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. This is an author produced version of a paper published in Plant and Cell Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Fusarium wilt; Nitrogen; Plant–pathogen interactions; Plant primary metabolism |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Feb 2019 12:41 |
Last Modified: | 02 Nov 2019 01:39 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/pcp/pcy211 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:141897 |