Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Metadata

Item Type:	Article
Authors/Creators:	Thery, C Witwer, KW Aikawa, E Jose Alcaraz, M Anderson, JD Andriantsitohaina, R Antoniou, A Arab, T Archer, F Atkin-Smith, GK Ayre, DC Bach, J-M Bachurski, D Baharvand, H Balaj, L Baldacchino, S Bauer, NN Baxter, AA Bebawy, M Beckham, C Zavec, AB Benmoussa, A Berardi, AC Bergese, P Bielska, E Blenkiron, C Bobis-Wozowicz, S Boilard, E Boireau, W Bongiovanni, A Borras, FE Bosch, S Boulanger, CM Breakefield, X Breglio, AM Brennan, MA Brigstock, DR Brisson, A Broekman, MLD Bromberg, JF Bryl-Gorecka, P Buch, S Buck, AH Burger, D Busatto, S Buschmann, D Bussolati, B Buzas, EI Byrd, JB Camussi, G Carter, DRF Caruso, S Chamley, LW Chang, Y-T Chen, C Chen, S Cheng, L Chin, AR Clayton, A Clerici, SP Cocks, A Cocucci, E Coffey, RJ Cordeiro-da-Silva, A Couch, Y Coumans, FAW Coyle, B Crescitelli, R Criado, MF D'Souza-Schorey, C Das, S Chaudhuri, AD de Candia, P De Santana Junior, EF De Wever, O del Portillo, HA Demaret, T Deville, S Devitt, A Dhondt, B Di Vizio, D Dieterich, LC Dolo, V Dominguez Rubio, AP Dominici, M Dourado, MR Driedonks, TAP Duarte, FV Duncan, HM Eichenberger, RM Ekstrom, K Andaloussi, SEL Elie-Caille, C Erdbrugger, U Falcon-Perez, JM Fatima, F Fish, JE Flores-Bellver, M Forsonits, A Frelet-Barrand, A Fricke, F Fuhrmann, G Gabrielsson, S Gamez-Valero, A Gardiner, C Gaertner, K Gaudin, R Gho, YS Giebel, B Gilbert, C Gimona, M Giusti, I Goberdhan, DCI Goergens, A Gorski, SM Greening, DW Gross, JC Gualerzi, A Gupta, GN Gustafson, D Handberg, A Haraszti, RA Harrison, P Hegyesi, H Hendrix, A Hill, AF Hochberg, FH Hoffmann, KF Holder, B Holthofer, H Hosseinkhani, B Hu, G Huang, Y Huber, V Hunt, S https://orcid.org/0000-0001-5572-1030 Ibrahim, AG-E Ikezu, T Inal, JM Isin, M Ivanova, A Jackson, HK Jacobsen, S Jay, SM Jayachandran, M Jenster, G Jiang, L Johnson, SM Jones, JC Jong, A Jovanovic-Talisman, T Jung, S Kalluri, R Kano, S-I Kaur, S Kawamura, Y Keller, ET Khamari, D Khomyakova, E Khvorova, A Kierulf, P Kim, KP Kislinger, T Klingeborn, M II, KDJ Kornek, M Kosanovic, MM Kovacs, AF Kraemer-Albers, E-M Krasemann, S Krause, M Kurochkin, IV Kusuma, GD Kuypers, S Laitinen, S Langevin, SM Languino, LR Lannigan, J Lasser, C Laurent, LC Lavieu, G Lazaro-Ibanez, E Le Lay, S Lee, M-S Lee, YXF Lemos, DS Lenassi, M Leszczynska, A Li, ITS Liao, K Libregts, SF Ligeti, E Lim, R Lim, SK Line, A Linnemannstoens, K Llorente, A Lombard, CA Lorenowicz, MJ Lorincz, AM Lotvall, J Lovett, J Lowry, MC Loyer, X Lu, Q Lukomska, B Lunavat, TR Maas, SLN Malhi, H Marcilla, A Mariani, J Mariscal, J Martens-Uzunova, ES Martin-Jaular, L Martinez, MC Martins, VR Mathieu, M Mathivanan, S Maugeri, M McGinnis, LK McVey, MJ Jr, MDG Meehan, KL Mertens, I Minciacchi, VR Moller, A Jorgensen, MM Morales-Kastresana, A Morhayim, J Mullier, F Muraca, M Musante, L Mussack, V Muth, DC Myburgh, KH Najrana, T Nawaz, M Nazarenko, I Nejsum, P Neri, C Neri, T Nieuwland, R Nimrichter, L Nolan, JP Nolte-'t Hoen, ENM Hooten, NN O'Driscoll, L O'Grady, T O'Loghlen, A Ochiya, T Olivier, M Ortiz, A Ortiz, LA Osteikoetxea, X Ostegaard, O Ostrowski, M Park, J Pegtel, DM Peinado, H Perut, F Pfaffl, MW Phinney, DG Pieters, BCH Pink, RC Pisetsky, DS von Strandmann, EP Polakovicova, I Poon, IKH Powell, BH Prada, I Pulliam, L Quesenberry, P Radeghieri, A Raffai, RL Raimondo, S Rak, J Ramirez, MI Raposo, G Rayyan, MS Regev-Rudzki, N Ricklefs, FL Robbins, PD Roberts, DD Rodrigues, SC Rohde, E Rome, S Rouschop, KMA Rughetti, A Russell, AE Saa, P Sahoo, S Salas-Huenuleo, E Sanchez, C Saugstad, JA Saul, MJ Schiffelers, RM Schneider, R Schoyen, TH Scott, A Shahaj, E Sharma, S Shatnyeva, O Shekari, F Shelke, GV Shetty, AK Shiba, K Siljander, PR-M Silva, AM Skowronek, A II, SOL Soares, RP Sodar, BW Soekmadji, C Sotillo, J Stahl, PD Stoorvogel, W Stott, SL Strasser, EF Swift, S Tahara, H Tewari, M Timms, K Tiwari, S Tixeira, R Tkach, M Toh, WS Tomasini, R Torrecilhas, AC Pablo Tosar, J Toxavidis, V Urbanelli, L Vader, P van Balkom, BWM van der Grein, SG Van Deun, J van Herwijnen, MJC Van Keuren-Jensen, K van Niel, G van Royen, ME van Wijnen, AJ Helena Vasconcelos, M Jr, VIJ Veit, TD Vella, LJ Velot, E Verweij, FJ Vestad, B Vinas, JL Visnovitz, T Vukman, KV Wahlgren, J Watson, DC Wauben, MHM Weaver, A Webber, JP Weber, V Wehman, AM Weiss, DJ Welsh, JA Wendt, S Wheelock, AM Wiener, Z Witte, L Wolfram, J Xagorari, A Xander, P Xu, J Yan, X Yanez-Mo, M Yin, H Yuana, Y Zappulli, V Zarubova, J Zekas, V Zhang, J-Y Zhao, Z Zheng, L Zheutlin, AR Zickler, AM Zimmermann, P Zivkovic, AM Zocco, D Zuba-Surma, EK
Copyright, Publisher and Additional Information:	© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords:	extracellular vesicles; exosomes; ectosomes; microvesicles; minimal information requirements; guidelines; standardization; microparticles; rigor; reproducibility
Dates:	Accepted: 25 September 2018 Published (online): 23 November 2018 Published: 23 November 2018
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield)
Depositing User:	Symplectic Sheffield
Date Deposited:	15 Mar 2019 10:26
Last Modified:	15 Mar 2019 10:26
Status:	Published
Publisher:	Taylor & Francis Open
Refereed:	Yes
Identification Number:	10.1080/20013078.2018.1535750
Related URLs:	Author
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:140343

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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

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