Agnoli, K., Haldipurkar, S.S., Tang, Y. et al. (2 more authors) (2019) Distinct modes of promoter recognition by two iron starvation σ factors with overlapping promoter specificities. Journal of Bacteriology, 201 (3). ISSN 0021-9193
Abstract
OrbS and PvdS are extracytoplasmic function (ECF) σ factors that regulate transcription of operons required for the biosynthesis of the siderophores ornibactin and pyoverdine in the B. cepacia complex and Pseudomonas spp., respectively. Here we show that promoter recognition by OrbS requires specific tetrameric -35 and -10 element sequences that are strikingly similar to those of the consensus PvdS-dependent promoter. However, whereas P. aeruginosa PvdS can serve OrbS-dependent promoters, OrbS cannot utilise PvdS-dependent promoters. To identify features present at OrbS-dependent promoters that facilitate recognition by OrbS, we carried out a detailed analysis of the nucleotide sequence requirements for promoter recognition by both OrbS and PvdS. This revealed that DNA sequence features located outside of the sigma binding elements are required for efficient promoter utilisation by OrbS. In particular, the presence of an A-tract extending downstream from the -35 element at OrbS-dependent promoters was shown to be an important contributor to OrbS specificity. Our observations demonstrate that the nature of the spacer sequence can have a major impact on promoter recognition by some ECF sigma factors through modulation of the local DNA architecture.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 American Society for Microbiology. This is an author produced version of a paper subsequently published in Journal of Bacteriology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Burkholderia cenocepacia; Pseudomonas aeruginosa; sigma factor; OrbS; PvdS; promoter; recognition; gene regulation; siderophore |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 Dec 2018 12:49 |
Last Modified: | 24 Nov 2021 11:27 |
Status: | Published |
Publisher: | American Society for Microbiology |
Refereed: | Yes |
Identification Number: | 10.1128/JB.00507-18 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:140134 |