Singh, H., Sreedharan, S., Tiwari, R. et al. (5 more authors) (2018) A fluorescent chemodosimeter for organelle-specific imaging of nucleoside polyphosphate dynamics in living cells. Crystal Growth and Design, 18 (11). pp. 7199-7206. ISSN 1528-7483
Abstract
Nucleoside polyphosphates (NPPs) are mainly produced in mitochondria and used as a universal energy source for various cellular events. Although numerous fluorescent probes for adenosine triphosphate (ATP) have been reported, they are not ideally suited for live monitoring of the subtle variation of the mitochondrial ATP level. A new coumarin-based fluorescent probe is synthesized, and this reagent is utilized for specific recognition of NPPs in mitochondria by super-resolution microscopy in physiological condition. Detailed 31P NMR studies reveal that the probe, L·Zn(II), binds to NPPs through their pendent phosphate functionalities. Such binding leads to a substantial enhancement in the luminescence intensity of L·Zn(II) + ADP or L·Zn(II) + ATP as compared to L·Zn(II). This—as well as 1H NMR spectroscopy—has enabled us to evaluate the probe’s binding affinities to these NPPs. Structured illumination and wide field fluorescence microscopy confirmed that this physiologically benign reagent is localized within mitochondria of live RAW 264.7 macrophage cells. This reagent has been utilized to probe real-time changes in ATP concentrations within mitochondria during drug-induced apoptosis.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 American Chemical Society. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 11 Dec 2018 11:44 |
Last Modified: | 11 Dec 2018 11:44 |
Published Version: | https://doi.org/10.1021/acs.cgd.8b01409 |
Status: | Published |
Publisher: | American Chemical Society |
Refereed: | Yes |
Identification Number: | 10.1021/acs.cgd.8b01409 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:139859 |