Finegan, T.M., Na, D., Cammarota, C. et al. (6 more authors) (2018) Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium. EMBO Journal, 37 (23). e100072. ISSN 0261-4189
Abstract
We investigated the cell behaviors that drive morphogenesis of the Drosophila follicular epithelium during expansion and elongation of early‐stage egg chambers. We found that cell division is not required for elongation of the early follicular epithelium, but drives the tissue toward optimal geometric packing. We examined the orientation of cell divisions with respect to the planar tissue axis and found a bias toward the primary direction of tissue expansion. However, interphase cell shapes demonstrate the opposite bias. Hertwig's rule, which holds that cell elongation determines division orientation, is therefore broken in this tissue. This observation cannot be explained by the anisotropic activity of the conserved Pins/Mud spindle‐orienting machinery, which controls division orientation in the apical–basal axis and planar division orientation in other epithelial tissues. Rather, cortical tension at the apical surface translates into planar division orientation in a manner dependent on Canoe/Afadin, which links actomyosin to adherens junctions. These findings demonstrate that division orientation in different axes—apical–basal and planar—is controlled by distinct, independent mechanisms in a proliferating epithelium.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The Authors. This is an author produced version of a paper subsequently published in EMBO Journal. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Cell division; epithelia; morphogenesis; spindle orientation; tissue biology |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Mathematics and Statistics (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 04 Dec 2018 15:57 |
Last Modified: | 26 May 2019 00:40 |
Published Version: | https://doi.org/10.15252/embj.2018100072 |
Status: | Published |
Publisher: | EMBO Press |
Refereed: | Yes |
Identification Number: | 10.15252/embj.2018100072 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:139524 |