Schmidt, Romy, Fulda, Martin, Paul, Melanie et al. (11 more authors) (2018) Low-oxygen response is triggered by an ATP-dependent shift in oleoyl-CoA in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. E12101–E12110. ISSN 1091-6490
Abstract
Plant response to environmental stimuli involves integration of multiple signals. Upon low-oxygen stress, plants initiate a set of adaptive responses to circumvent an energy crisis. Here, we reveal how these stress responses are induced by combining (i) energy-dependent changes in the composition of the acyl-CoA pool and (ii) the cellular oxygen concentration. A hypoxia-induced decline of cellular ATP levels reduces LONG-CHAIN ACYL-COA SYNTHETASE activity, which leads to a shift in the composition of the acyl-CoA pool. Subsequently, we show that different acyl-CoAs induce unique molecular responses. Altogether, our data disclose a role for acyl-CoAs acting in a cellular signaling pathway in plants. Upon hypoxia, high oleoyl-CoA levels provide the initial trigger to release the transcription factor RAP2.12 from its interaction partner ACYL-COA BINDING PROTEIN at the plasma membrane. Subsequently, according to the N-end rule for proteasomal degradation, oxygen concentration-dependent stabilization of the subgroup VII ETHYLENE-RESPONSE FACTOR transcription factor RAP2.12 determines the level of hypoxia-specific gene expression. This research unveils a specific mechanism activating low-oxygen stress responses only when a decrease in the oxygen concentration coincides with a drop in energy.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 the Author(s) |
Keywords: | ACBP,Acyl-CoA,ERFVII,Integrative signaling,Low-oxygen stress,Diazepam Binding Inhibitor/metabolism,Signal Transduction,Arabidopsis/genetics,Stress, Physiological,Adenosine Triphosphate/metabolism,Oxygen/metabolism,Cell Hypoxia,Models, Biological,Acyl Coenzyme A/metabolism,Gene Expression Regulation, Plant,Arabidopsis Proteins/metabolism |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York) |
Depositing User: | Pure (York) |
Date Deposited: | 04 Dec 2018 17:00 |
Last Modified: | 08 Feb 2025 00:32 |
Published Version: | https://doi.org/10.1073/pnas.1809429115 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1073/pnas.1809429115 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:139488 |
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