Cressiot, Benjamin, Greive, Sandra Joy Muriatu orcid.org/0000-0001-6067-2632, Mojtabavi, Mehrnaz et al. (2 more authors) (2018) Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors. Nature Communications. 4652. pp. 1-7. ISSN 2041-1723
Abstract
Nanopore-based sensors are advancing the sensitivity and selectivity of single-molecule detection in molecular medicine and biotechnology. Current electrical sensing devices are based on either membrane protein pores supported in planar lipid bilayers or solid-state (SS) pores fabricated in thin metallic membranes. While both types of nanosensors have been used in a variety of applications, each has inherent disadvantages that limit its use. Hybrid nanopores, consisting of a protein pore supported within a SS membrane, combine the robust nature of SS membranes with the precise and simple engineering of protein nanopores. We demonstrate here a novel lipid-free hybrid nanopore comprising a natural DNA pore from a thermostable virus, electrokinetically inserted into a larger nanopore supported in a silicon nitride membrane. The hybrid pore is stable and easy to fabricate, and, most importantly, exhibits low peripheral leakage allowing sensing and discrimination among different types of biomolecules.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2018, The Author(s). |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 12 Nov 2018 15:40 |
Last Modified: | 05 Jan 2025 00:19 |
Published Version: | https://doi.org/10.1038/s41467-018-07116-x |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-018-07116-x |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:138535 |