Al-Shammari, AA, Kissane, RWP, Holbeck, S et al. (5 more authors) (2019) Integrated method for quantitative morphometry and oxygen transport modelling in striated muscle. Journal of Applied Physiology, 126 (3). pp. 544-557. ISSN 8750-7587
Abstract
Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2018, Journal of Applied Physiology. This is an author produced version of a paper published in Journal of Applied Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Mathematical Modelling; Skeletal Muscle; Oxygen Transport; Fiber Type Composition; Capillary Supply |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Oct 2018 13:54 |
Last Modified: | 06 Dec 2019 01:38 |
Status: | Published |
Publisher: | American Physiological Society |
Identification Number: | 10.1152/japplphysiol.00170.2018 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:137570 |