Proctor, M.S., Chidgey, J.W., Shukla, M.K. et al. (4 more authors) (2018) Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase. FEBS Letters , 592 (18). pp. 3062-3073. ISSN 1873-3468
Abstract
In the model cyanobacteriumSynechocystissp. PCC 6803, the terminalenzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms acomplex with high light-inducible proteins, the photosystem II assembly fac-tor Ycf39 and the YidC/Alb3/OxaI membrane insertase, co-ordinatingchlorophyll delivery with cotranslational insertion of nascent photosystempolypeptides into the membrane. To gain insight into the ubiquity of thisassembly complex in higher photosynthetic organisms, we produced functionalforeign chlorophyll synthases in a cyanobacterial host. Synthesis of algal andplant chlorophyll synthases allowed deletion of the otherwise essential nativecyanobacterial gene. Analysis of purified protein complexes shows that theinteraction with YidC is maintained for both eukaryotic enzymes, indicatingthat a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algaeand plants.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Arabidopsis; chlorophyll; chlorophyll synthase; cyanobacteria; high light-inducible proteins; YidC/Alb3/OxaI |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Oct 2018 10:30 |
Last Modified: | 22 Oct 2018 10:30 |
Published Version: | https://doi.org/10.1002/1873-3468.13222 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/1873-3468.13222 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:137433 |