Owen, H.J., Sun, R., Ahmad, A. et al. (4 more authors) (2018) TssA from Burkholderia cenocepacia: expression, purification, crystallization and crystallographic analysis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 74 (9). pp. 536-542. ISSN 2053-230X
Abstract
TssA is a core component of the type VI secretion system, and phylogenetic analysis of TssA subunits from different species has suggested that these proteins fall into three distinct clades. Whilst representatives of two clades, TssA1 and TssA2, have been the subjects of investigation, no members of the third clade (TssA3) have been studied. Constructs of TssA from Burkholderia cenocepacia, a representative of clade 3, were expressed, purified and subjected to crystallization trials. Data were collected from crystals of constructs of the N-terminal and C-terminal domains. Analysis of the data from the crystals of these constructs and preliminary structure determination indicates that the C-terminal domain forms an assembly of 32 subunits in D16 symmetry, whereas the N-terminal domain is not involved in subunit assocation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
Keywords: | type VI secretion system; TssA; Burkholderia cenocepacia |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Oct 2018 11:58 |
Last Modified: | 15 Oct 2018 11:58 |
Published Version: | https://doi.org/10.1107/S2053230X18009706 |
Status: | Published |
Publisher: | International Union of Crystallography |
Identification Number: | 10.1107/S2053230X18009706 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:137026 |