Matharu, Avtar Singh orcid.org/0000-0002-9488-565X, Melo, Eduardo MacEdo De, Grudniewska, Aleksandra et al. (3 more authors) (2018) Enhanced Protein Extraction From Oilseed Cakes Using Glycerol-Choline Chloride Deep Eutectic Solvents:A Biorefinery Approach. ACS Sustainable Chemistry & Engineering. ISSN 2168-0485
Abstract
The enhanced extraction of proteins from rapeseed cake (RC) and evening primrose cake (EC) using a glycerol-choline chloride deep eutectic solvent (Glyceline) is reported. Protein-rich precipitates were obtained by adding water (antisolvent) to the DES extract derived at different processing temperatures. The presence of proteins in precipitates has been confirmed by several techniques, such as: NMR, ATR-IR, TGA, CHN and SDS-PAGE. Yield of precipitates improved with increasing temperature of treatment, reaching a maximum of 20% and 35% at 140 °C from RC and EC, respectively. In general, the protein content of the extracts was ca. 40–50%, which is up to 20% more than the starting materials. SDS-PAGE confirmed that glyceline selectively extracted cruciferin proteins (ca. 16–33 kDa) from RC, while proteins with variable molecular weight (10–40 kDa) were identified in EC extracts. As potential application cruciferin-rich RCP60 and RCP100 could be incorporated into final food formulations as a source of protein due to its light colour
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 05 Oct 2018 08:50 |
Last Modified: | 12 Jan 2025 00:06 |
Status: | Published online |
Refereed: | Yes |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:136752 |
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