Vernoux, T, Brunoud, G, Farcot, E et al. (21 more authors) (2011) The auxin signalling network translates dynamic input into robust patterning at the shoot apex. Molecular Systems Biology, 7 (1). 508. ISSN 1744-4292
Abstract
The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin response factors (ARFs) and Aux/IAAs. Here, we perform a large‐scale analysis of the Aux/IAA‐ARF pathway in the shoot apex of Arabidopsis, where dynamic auxin‐based patterning controls organogenesis. A comprehensive expression map and full interactome uncovered an unexpectedly simple distribution and structure of this pathway in the shoot apex. A mathematical model of the Aux/IAA‐ARF network predicted a strong buffering capacity along with spatial differences in auxin sensitivity. We then tested and confirmed these predictions using a novel auxin signalling sensor that reports input into the signalling pathway, in conjunction with the published DR5 transcriptional output reporter. Our results provide evidence that the auxin signalling network is essential to create robust patterns at the shoot apex.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2011, EMBO and Macmillan Publishers Limited. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License (CC BY-NC-ND 3.0), which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. https://creativecommons.org/licenses/by-nc-nd/3.0/ |
Keywords: | auxin; biosensor; live imaging; ODE; signalling |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 16 May 2019 10:51 |
Last Modified: | 16 May 2019 10:51 |
Status: | Published |
Publisher: | Wiley Open Access |
Identification Number: | 10.1038/msb.2011.39 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:136261 |
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