Khamoui, AV, Desai, M, Ross, MG et al. (1 more author) (2018) Sex-specific effects of maternal and postweaning high-fat diet on skeletal muscle mitochondrial respiration. Journal of Developmental Origins of Health and Disease, 9 (6). pp. 670-677. ISSN 2040-1744
Abstract
Exposure to maternal over-nutrition in utero is linked with developmental programming of obesity, metabolic syndrome and cardiovascular disease in offspring, which may be exacerbated by postnatal high-fat (HF) diet. Skeletal muscle mitochondrial function contributes to substrate metabolism and is impaired in metabolic disease. We examined muscle mitochondrial respiration in male and female mice exposed to maternal HF diet in utero, followed by postweaning HF diet until middle age. After in utero exposure to maternal control (Con) or HF diet (45% kcal fat; 39.4% lard, 5.5% soybean oil), offspring were weaned to Con or HF, creating four groups: Con/Con (male/female (m/f), n=8/8), Con/HF (m/f, n=7/4), HF/Con (m/f, n=9/6) and HF/HF (m/f, n=4/4). Oxidative phosphorylation (OXPHOS) and electron transfer system (ETS) capacity were measured in permeabilized gastrocnemius bundles. Maternal HF diet increased fasting glucose and lean body mass in males and body fat percentage in both sexes (P⩽0.05). Maximal adenosine diphosphate-stimulated respiration (complex I OXPHOS) was decreased by maternal HF diet in female offspring (−21%, P=0.053), but not in male (−0%, P>0.05). Sexually divergent responses were exacerbated in offspring weaned to HF diet. In females, OXPHOS capacity was lower (−28%, P=0.041) when weaned to high-fat (HF/HF) v. control diet (HF/Con). In males, OXPHOS (+33%, P=0.009) and ETS (+42%, P=0.016) capacity increased. Our data suggest that maternal lard-based HF diet, rich in saturated fat, affects offspring skeletal muscle respiration in a sex-dependent manner, and these differences are exacerbated by HF diet in adulthood.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2018. This article has been published in a revised form in Journal of Developmental Origins of Health and Disease https://doi.org/10.1017/S2040174418000594. This version is free to view and download for private research and study only. Not for re-distribution, re-sale or use in derivative works. |
Keywords: | developmental programming; fetal programming; oxidative phosphorylation; respirometry; sexual dimorphism |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Sep 2018 12:38 |
Last Modified: | 14 Feb 2019 15:44 |
Status: | Published |
Publisher: | Cambridge University Press |
Identification Number: | 10.1017/S2040174418000594 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:136165 |