Marklew, C.J., Booth, A., Beales, P.A. et al. (1 more author) (2018) Membrane remodelling by a lipidated endosomal sorting complex required for transport-III chimera, in vitro. Interface Focus, 8 (5). 20180035. ISSN 2042-8898
Abstract
The complexity of eukaryotic cells is underscored by the compartmentalization of chemical signals by phospholipid membranes. A grand challenge of synthetic biology is building life from the ‘bottom-up’, for the purpose of generating systems simple enough to precisely interrogate biological pathways or for adapting biology to perform entirely novel functions. Achieving compartmentalization of chemistries in an addressable manner is a task exquisitely refined by nature and embodied in a unique membrane remodelling machinery that pushes membranes away from the cytosol, the ESCRT-III (endosomal sorting complex required for transport-III) complex. Here, we show efforts to engineer a single ESCRT-III protein merging functional features from its different components. The activity of such a designed ESCRT-III is shown by its ability to drive the formation of compartments encapsulating fluorescent cargo. It appears that the modular nature of ESCRT-III allows its functional repurposing into a minimal machinery that performs sophisticated membrane remodelling, therefore enabling its use to create eukaryotic-like multi-compartment architectures.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The Author(s). This is an author produced version of a paper subsequently published in Interface Focus. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Membranes; ESCRT; compartmentalization; artificial cells |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) UNSPECIFIED ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/M027821/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Sep 2018 09:35 |
Last Modified: | 27 Sep 2018 05:50 |
Published Version: | https://doi.org/10.1098/rsfs.2018.0035 |
Status: | Published |
Publisher: | The Royal Society |
Refereed: | Yes |
Identification Number: | 10.1098/rsfs.2018.0035 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:135952 |